Anti-γ-Tubulin Antibody(monoclonal, GTU-88）

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分子生物学
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品名：Anti-γ-Tubulin Antibody(monoclonal, GTU-88）
品牌：博士德
货号：BM1606

货号：BM1606

应用：WB, IHC-P

反应性：Human, Mouse, Rat

克隆性：内参抗体

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SKU	包装规格	单位	目录价	您的价格	库存
BM1606-100ul	100ul	EA	¥1780.00	请登录	请登录
BM1606-150ul	150ul	EA	¥2580.00	请登录	请登录
BM1606-50ul	50ul	EA	¥1060.00	请登录	请登录

产品概况

货号	BM1606
产品名称	Anti-γ-Tubulin Antibody(monoclonal, GTU-88）
基因名	GGA1; GGA2; GGA3
抗体来源	Mouse
克隆	Monoclonal (Clone: GTU-88)
抗体亚型	Mouse IgG1
分子量	55KD
免疫原	Synthetic gamma-tubulin peptide(amino acids 38-53), conjugated to KLH.
内容	200 ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol.
纯化方式	Ascites
浓度	200ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	Gamma-tubulin is a universal component of microtubule organizing centers, which is essential for nuclear division and microtubule assembly in Aspergillus nidulans. TUBG1 mapped to within 20 kb of TUBG2 at 17q21. Gamma-tubulin is a minor protein, present at less than 1% the level of alpha- and beta-tubulin, and is limited to the centrosome. Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens.
研究类别	1. Rommens, J. M.; Durocher, F.; McArthur, J.; Tonin, P.; LeBlanc, J.-F.; Allen, T.; Samson, C.; Ferri, L.; Narod, S.; Morgan, K.; Simard, J. : Generation of a transcription map at the HSD17B locus centromeric to BRCA1 at 17q21. Genomics 28: 530-542, 1995. 2. Zheng, Y.; Jung, M. K.; Oakley, B. R. : Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome. Cell 65: 817-823, 1991. 3. Stearns, T.; Evans, L.; Kirschner, M. : Gamma-tubulin is a highly conserved component of the centrosome. Cell 65: 825-836, 1991.
Uniprot ID	GGA1; GGA2; GGA3: Q9UJY5
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

产品应用细节

为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot:	1:500-2000
Immunohistochemistry in paraffin section IHC-(P):	1:50-400

*最优稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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[list_product_images]Figure 1. Western blot analysis of anti-TUBG1 antibody (BM1606). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat brain tissue lysates,Lane 2: human HELA whole cell lysates,Lane 3: human COLO-320 whole cell lysates,Lane 4: mouse HEPA whole cell lysates.Use rabbit anti- TUBG1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for TUBG1 at approximately 55KD. The expected band size for TUBG1 is at 55KD.|Figure 2.IHC analysis using anti-TUBG1 antibody (BM1606). detected in paraffin-embedded section of ?rat intestinal tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3.IHC analysis using anti-TUBG1 antibody (BM1606). detected in paraffin-embedded section of ?mouse intestinal tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.[/list_product_images]

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