Product Overview

The Multiplex Human MMP (6-plex) is a fully quantitative ELISA-based chemiluminescent assay. This kit allows for the concurrent measurement of five matrix metalloproteinases (MMPs). MMPs are an important enzyme in cell biology, involved in cleaving extracellular matrix and cell surface receptor proteins (such as cytokines, chemokines, and apoptotic ligands). The specific markers/analytes included in this kit are MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, and MMP-13.

Using just 50 µl of sample per well, up to 80 samples can be assayed for five MMPs in approximately 5.5 hours. Multiplex Arrays provide researchers with an easy-to-use and cost-effective means of generating an MMP profile for each sample.

Multiplex plates are built by adsorbing multiple distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Assay Ranges

Kit Components

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.

Reagents Include:

Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (20): Adhesive strips