Anti-VDAC1 Antibody

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品名：Anti-VDAC1 Antibody
品牌：博士德
货号：BA3754

货号：BA3754

应用：WB, IHC-P, IHC-F, ICC

反应性：Human, Mouse, Rat

克隆性：内参抗体

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SKU	包装规格	单位	目录价	您的价格	库存
BA3754-100ul	100ul	EA	¥1780.00	请登录	请登录
BA3754-150ul	150ul	EA	¥2580.00	请登录	请登录
BA3754-50ul	50ul	EA	¥1060.00	请登录	请登录

产品概况

货号	BA3754
产品名称	Anti-VDAC1 Antibody
基因名	VDAC1
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit IgG
分子量	31KD
免疫原	A synthetic peptide corresponding to a sequence in the middle region of human VDAC(163-178aa RVTQSNFAVGYKTDEF), identical to the related rat and mouse sequences.
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	Immunogen affinity purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	The voltage-dependent anion channel(VDAC) of the outer mitochondrial membrane is a small, abundant outer membrane pore-forming protein found in the outer membranes of all eukaryotic mitochondria. The VDAC protein is though to form the major pathway for movement of adenine nucleotides through the outer membrane and to be the mitochondrial binding site for hexokinase and glycerol kinase. At low transmembrane voltage, VDAC is open for anions such as phosphate, chloride, and adenine nucleotides. At higher transmembrane voltage, VDAC functions as a selective channel for cations and uncharged molecules. These features make VDAC likely to play a role in mitochondrial energy metabolism. Huizing et al. studied by Northern and Western blot analyses the human tissue distribution of mitochondrial transmembrane metabolite carriers. They found that VDAC1 mRNA has a ubiquitous distribution, with most pronounced expression in heart, liver, and skeletal muscle, whereas the VDAC2 isoform appears to be expressed only in the heart.
研究类别	1. Blachly-Dyson, E., Zambronicz, E. B., Yu, W. H., Adams, V., McCabe, E. R. B., Adelman, J., Colombini, M., Forte, M.Cloning and functional expression in yeast of two human isoforms of the outer mitochondrial membrane channel, the voltage-dependent anion channel.J. Biol. Chem. 268: 1835-1841, 1993.2. Geisler, S., Holmstrom, K. M., Skujat, D., Fiesel, F. C., Rothfuss, O. C., Kahle, P. J., Springer, W.PINK1/Parkin-mediated mitophagy is dependent on VDAC1 and p62/SQSTM1.Nature Cell Biol. 12: 119-131, 2010.3. Huizing, M., Ruitenbeek, W., van den Heuvel, L. P., Dolce, V., Iacobazzi, V., Smeitink, J. A. M., Palmieri, F., Trijbels, J. M. F.Human mitochondrial transmembrane metabolite carriers: tissue distribution and its implication for mitochondrial disorders.J. Bioenerg. Biomembr. 30: 277-284, 1998.
Uniprot ID	VDAC1: P21796
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。

产品应用细节

为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot:	1:500-2000
Immunohistochemistry(Paraffin-embedded Section):	1:50-400
Immunohistochemistry(Frozen Section):	1:50-400
Immunocytochemistry:	1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*最优稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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[list_product_images]Figure 1. Western blot analysis of Anti-VDAC1 antibody (BA3754). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat skeletal muscle tissue lysates,Lane 2: Rat heart tissue lysates,Lane 3: Rat liver tissue lysates,Lane 4: HELA whole cell lysates,Lane 5: A431 whole cell lysates,Lane 6: A549 whole cell lysates,Lane 7: SMMC whole cell lysates,Lane 8: HT1080 whole cell lysates,Use rabbit Anti-VDAC1 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for VDAC1 at approximately 31KD. The expected band size for VDAC1 is at 31KD.|Figure 2. IHC analysis using Anti-VDAC1 antibody (BA3754) detected in paraffin-embedded section of rat cardiac muscle tissue. Biotinylated goat Anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using Anti-VDAC1 antibody (BA3754) detected in paraffin-embedded section of rat skeletal muscle tissue. Biotinylated goat Anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis using Anti-VDAC1 antibody (BA3754) detected in paraffin-embedded section of NIH3T3 cell. Biotinylated goat Anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.[/list_product_images]

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