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分子生物学
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细胞生物学
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蛋白质组学
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蛋白凝胶纯化
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生化试剂
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仪器设备
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通用实验耗材
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色谱耗材
Product Info Summary
| Species: | Human |
|---|---|
| Sample Type: | Serum, EDTA Plasma |
| Volume Required per Well: | Min 25 µl |
| Assay Length: | 2.25 hours |
| Specificity: | All analytes < 1% intra-panel cross-reactivity |
| Assay Type: | Planar Based Multiplexed ELISA |
| Detection Method: | Chemiluminescent |
| Multiplex Format: | 96-well solid plate |
| Catalog: | MEK1010 |
| Manufacturer Reference Number: | 115233HU |
| Analytes: | IL-1β, IL-6, TNFα, IFN-γ, IL-1α, IL-10 |
Product Overview
The Multiplex Human Cytokine Panel 1 (6-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of six biomarkers or analytes (IL-1β, IL-6, TNFα, IFN-γ, IL-1α, IL-10).
Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing six distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Using just 50 µl of sample per well, up to 80 samples can be assayed for all six markers in the panel in 2.25 hours. Multiplex Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.
Assay Ranges
| Units | Calibrator Range | LLD Limit | |
| IL-1β | pg/ml | 10000 - 13.72 | 10.14 |
| IL-6 | pg/ml | 3000 - 4.11 | 3.47 |
| TNFα | pg/ml | 4000 - 5.49 | 5.20 |
| IFN-γ | pg/ml | 8000 - 10.97 | 4.95 |
| IL-1α | pg/ml | 4000 - 5.49 | 5.43 |
| IL-10 | pg/ml | 3000 - 4.11 | 4.10 |
Kit Components
Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.
Reagents Include:
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (20): Adhesive strips
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