Species: | Human |
Sample Type: | Whole Blood |
Volume Required per Well: | Min 15 µl |
Assay Length: | 3.5 hours |
Specificity: | All analytes < 1% intra-panel cross-reactivity |
Assay Type: | Planar Based Multiplexed ELISA |
Detection Method: | Chemiluminescent |
Multiplex Format: | 96-well solid plate |
SKU | MEK2008 |
Manufacturer Reference Number: | 565949HU |
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Analytes | HRP2, LDH-Pan, LDH-Pv, LDH-Pf, CRP |
The Multiplex ELISA Kit For Human Malaria (5-Plex) is a robust and fully quantitative chemiluminescent assay for the concurrent surveillance of four malaria biomarkers. The assay simultaneously measures HRP2, LDH-Pan, LDH-Pv, LDH-Pf, and one host biomarker, CRP. The monitoring of subclinical Malaria infections and prevalence of HRP2-deletions is achieved by the coupling of the most sensitive commercially available HPR2 assay with LDH detection. Validated protocols for whole blood samples are in place, with protocols for dry blood spots in development. In 3.5 hours, each kit will report quantities of 80 samples for the four Malaria biomarkers and one host biomarker CRP. This is achieved while only using 15 μl of whole blood per sample.
Units | Calibrator Range | LLD Limit | |
HRP2 | pg/mL | .7-2700 | 0.92 |
Pan-LDH | pg/mL | 20-75000 | 24 |
Pv-LDH | pg/mL | 5-20000 | 6 |
Pf-LDH | pg/mL | 6-23000 | 8 |
CRP | ng/mL | 11-45000 | 15 |
Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.
Reagents Include:
Calibrator
Detection Mix
Substrate
Sample Diluents
Streptavidin HRP
Wash Buffer
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