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产品概况
| 货号 | EK0303 |
|---|---|
| 产品名称 | Rat Activin A ELISA Kit |
| 产品描述 | For quantitative detection of rat Activin A in , serum, plasma(heparin, EDTA) and saliva. |
| 免疫原 | CHO, G311-S426 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <12pg/ml |
| 检测范围 | 15.6pg/ml-1000pg/ml |
| 基因名称 | INHBA |
| 基因全名 | inhibin, beta A |
| Uniprot ID | P18331 |
| 指标别名 | Activin beta A chain|erythroid differentiation factor|erythroid differentiation protein|follicle stimulating hormone releasing protein|FSH releasing protein|Inhibin|inhibin beta A chain |
| 背景资料 | Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa. |
| 显色时间 | 25-30分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0303-CAP | 96-well plate precoated with anti- rat Activin A antibody | 1 |
| EK0303-ST | Lyophilized recombinant rat Activin A standard | 10ng/tube×2 |
| EK0303-DA | Biotinylated anti- rat Activin A antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 31 | 151 | 450 | 28 | 138 | 453 |
| Standard deviation | 1.3 | 8 | 25.2 | 1.56 | 8.69 | 25.36 |
| CV(%) | 4.2% | 5.3% | 5.6% | 5.6% | 6.3% | 5.6% |
Rat Activin A ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 25-30分钟)| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.028 | 0.049 | 0.111 | 0.175 | 0.328 | 0.851 | 1.457 | 2.491 |
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