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分子生物学
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细胞生物学
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蛋白质组学
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蛋白凝胶纯化
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生化试剂
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仪器设备
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通用实验耗材
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色谱耗材
Product Info Summary
| Species | Human |
| Assay Type | Sandwich |
| Sample Type | Human Serum, EDTA/Heparin Plasma |
| Assay Length | 4.83 hours |
| Specificity | All analytes < 1% intra-panel cross-reactivity |
| Volume Required Per Well | Min 25µl |
| Detection Method | Chemiluminescent |
| Multiplex Format | 96-well solid plate |
| Catalog No | MEK2007 |
| Manufacturer Reference Number: | 119733HU |
|---|---|
| Analytes | IL-1b, IL-1Ra, IL-2, IL-2Ra, IL-6, IL-6r, IL-8, IL-10, IL12p70, IL-13, IFNy, IFNα, GM-CSF, TNFα, MCP-1, MIP1a |
Product Overview
The Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Using only 25 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 4.83 hours. The Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Assay Ranges
| Units | Calibrator Range | LLD Limit | |
| GM-CSF | pg/ml | 6,000-10.70 | 5.35 |
| IFNα | pg/ml | 400-0.72 | 0.36 |
| IFNγ | pg/ml | 99-0.18 | 0.09 |
| IL-1β | pg/ml | 4,075-7.27 | 3.64 |
| IL-1Ra | pg/ml | 7,000-12.48 | 6.24 |
| IL-2 | pg/ml | 64-0.11 | 0.06 |
| IL-2Ra | pg/ml | 16,000-28.5 | 14.25 |
| IL-6 | pg/ml | 333-0.59 | 0.3 |
| IL-6R | pg/ml | 1,250-2.23 | 1.11 |
| IL-8 | pg/ml | 2,000-3.6 | 1.8 |
| IL-10 | pg/ml | 2,680-4.78 | 2.39 |
| IL-12p70 | pg/ml | 3,000-5.35 | 2.68 |
| IL-13 | pg/ml | 333-0.59 | 0.3 |
| MCP-1 | pg/ml | 2,000-3.6 | 1.8 |
| MIP-1α | pg/ml | 2,000-3.57 | 1.78 |
| TNFα | pg/ml | 661-1.18 | 0.59 |
Kit Components
Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing.
Reagents Include:
Calibrator
Detection Mix
Substrate
Sample Diluents
Streptavidin HRP
Wash Buffer
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