Anti-GAPDH Antibody

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品名：Anti-GAPDH Antibody
品牌：博士德
货号：BA2913

货号：BA2913

应用：WB, IHC-P

反应性：Human,Mouse,Rat

克隆性：内参抗体

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SKU	包装规格	单位	目录价	您的价格	库存
BA2913-100ul	100ul	EA	¥1780.00	请登录	请登录
BA2913-150ul	150ul	EA	¥2580.00	请登录	请登录
BA2913-50ul	50ul	EA	¥1060.00	请登录	请登录

产品概况

货号	BA2913
产品名称	Anti-GAPDH Antibody
基因名	GAPDH
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit IgG
分子量	36KD
免疫原	A synthetic peptide corresponding to a sequence at the N-terminus of human GAPDH(30-44aa IVAINDPFIDLNYMV), identical to the related mouse and rat sequences.
内容	500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA
纯化方式	Immunogen affinity purified.
浓度	500ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
背景资料	Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide(NAD). The enzyme is though to be a tetramer of identical chains. Several highly homologous glyceraldehyde-3-phosphate dehydrogenase(GAPD)-related sequences have been identified previously in human DNA by Southern blot analysis. Protein studies have identified only a single expressed locus for this major glycolytic enzyme, and this maps to chromosome 12p13. Glyceraldehyde-3-phosphate dehydrogenase(GAPDH) is a critical regulator of CICD, it mediates an elevation in glycolysis and enhanced autophagy that cooperate to protect cells from CICD.
Uniprot ID	GAPDH: P04406

产品应用细节

为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot:	1:500-2000
Immunohistochemistry(Paraffin-embedded Section):	1:50-400
Immunohistochemistry(Frozen Section):	1:50-400
Immunocytochemistry:	1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*最优稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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[list_product_images]Figure 1. Western blot analysis of anti- GAPDH Antibody (BA2913). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat myocardium tissue lysates,
Lane 2: brain tissue lysates.
Use rabbit anti- GAPDH 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for GAPDH at approximately 36KD. The expected band size for GAPDH is at 36KD.|Figure 2. ICC analysis using anti- GAPDH Antibody (BA2913). was detected in immersion fixed SMMC cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)Catalog # BA1127) and counterstained with DAPI (blue). [/list_product_images]

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