Anti-β-tubulin Antibody(monoclonal, TUB 2.1）

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品名：Anti-β-tubulin Antibody(monoclonal, TUB 2.1）
品牌：博士德
货号：BM1453

货号：BM1453

应用：WB, IHC-P, ICC/IF

反应性：Human, Mouse, Rat

克隆性：内参抗体

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SKU	包装规格	单位	目录价	您的价格	库存
BM1453-100ul	100ul	EA	¥1780.00	请登录	请登录
BM1453-150ul	150ul	EA	¥2580.00	请登录	请登录
BM1453-50ul	50ul	EA	¥1060.00	请登录	请登录

产品概况

货号	BM1453
产品名称	Anti-β-tubulin Antibody(monoclonal, TUB 2.1）
基因名	TUBB1
抗体来源	Mouse
克隆	Monoclonal (Clone: TUB 2.1)
抗体亚型	Mouse IgG1
分子量	55KD
免疫原	Tubulin from rat brain.
内容	200 ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol.
纯化方式	Ascites
浓度	200ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	Tubulin beta(TUBB) was derived from its corresponding mRNA with a molecular weight of about 55,000. TUBB gene contains 4 exons and located in the HLA class I region at 6p21.3. Paclitaxel resistance in non-small-cell lung cancer associated with beta-tubulin gene mutations.
研究类别	1. Floyd-Smith, G. A.; de Martinville, B.; Francke, U. : A beta-tubulin expressed gene M40 (TUBB) is located on human chromosome 6 and two related pseudogenes are located on chromosomes 8 (TUBBP1) and 13 (TUBBP2). (Abstract) Cytogenet. Cell Genet. 40: 629, 1985. 2. Floyd-Smith, G. A.; de Martinville, B.; Francke, U. : An expressed beta-tubulin gene, TUBB, is located on the short arm of human chromosome 6 and two related sequences are dispersed on chromosomes 8 and 13. Exp. Cell Res. 163: 539-548, 1986.
Uniprot ID	TUBB1: Q9H4B7
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

产品应用细节

为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot:	1:500-2000
Immunohistochemistry(Paraffin-embedded Section):	1:50-400
Immunocytochemistry/Immunofluorescence (ICC/IF):	1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*最优稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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[list_product_images]WB: Rat Skeletal Muscle Tissue Lysate|IHC(P): Human Mammary Cancer Tissue|Figure 3.ICC analysis using anti-TUBB1 antibody (BM1453) was detected in immersion fixed A549 cell line . Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)Catalog#BA1126) and counterstained with DAPI (blue).[/list_product_images]

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