Anti-β-Actin Antibody

货号:BM3873
应用:WB
反应性:Human, Mouse, Rat, Zebrafish, Monkey
克隆性:内参抗体
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BM3873-100ul 100ul EA ¥880.00 请登录 请登录
BM3873-1ml 1ml EA ¥6800.00 请登录 请登录
BM3873-200ul 200ul EA ¥1680.00 请登录 请登录

产品概况

货号BM3873
产品名称Anti-β-Actin Antibody
基因名ACTB
抗体来源Rabbit
克隆Monoclonal
抗体亚型Rabbit
分子量42KD
免疫原A synthesized peptide derived from human beta Actin
内容500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式Immunogen affinity purified.
浓度500 ug/ml
产品形态Liquid
保存条件12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.
Uniprot IDACTB: P60709
推荐配套的二抗和检测试剂Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

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[list_product_images]Figure 1. Western blot analysis of anti- β-Actin antibody (BM3873).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Human HELA whole cell lysates,Lane 2: Human HepG2 whole cell lysates,Lane 3: Monkey kidney tissue lysates,Lane 4: Monkey liver tissue lysates,Lane 5: Rat heart tissue lysates,Lane 6: Rat brain tissue lysates,Lane 7: Rat PC-12 whole cell lysates,Lane 8: Mouse heart tissue lysates,Lane 9: Mouse brain tissue lysates,Lane 10: Mouse ANA-1 whole cell lysates.Use rabbit anti- β-Actin 1:3000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for β-Actin at approximately 42KD. The expected band size for β-Actin is at 42KD.[/list_product_images]
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