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分子生物学
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细胞生物学
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蛋白质组学
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蛋白凝胶纯化
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生化试剂
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仪器设备
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通用实验耗材
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色谱耗材
Product Info Summary
| Species: | Human |
|---|---|
| Sample Type: | Serum, EDTA Plasma |
| Volume Required per Well: | Min 25 µl |
| Assay Length: | 2.25 hours |
| Specificity: | All analytes < 1% intra-panel cross-reactivity |
| Assay Type: | Planar Based Multiplexed ELISA |
| Detection Method: | Chemiluminescent |
| Multiplex Format: | 96-well solid plate |
| Catalog: | MEK1008 |
| Manufacturer Reference Number: | 110433HU |
| Analytes: | IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IFNγ, TNFα |
Product Overview
The Multiplex Human Cytokine HS (9-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 9 biomarkers or analytes. Using less than 50 µl of sample per well, up to 80 samples can be assayed for all 9 markers in the panel within 2.25 hours. The Multiplex Human Cytokine HS (9-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 9 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Assay Ranges
| Units | Calibrator Range | LLD Limit | |
| IL-1α | pg/ml | 4000 – 5.49 | 5.43 |
| IL-1β | pg/ml | 10000 – 13.72 | 10.14 |
| IL-2 | pg/ml | 4000 – 5.49 | 3.16 |
| IL-4 | pg/ml | 2000 – 2.74 | 2.14 |
| IL-6 | pg/ml | 3000 – 4.11 | 3.47 |
| IL-8 | pg/ml | 2000 – 2.74 | 2.70 |
| IL-10 | pg/ml | 3000 – 4.11 | 4.10 |
| IFNγ | pg/ml | 8000 – 10.97 | 4.95 |
| TNFα | pg/ml | 4000 – 5.49 | 5.20 |
Kit Components
Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.
Reagents Include:
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (20): Adhesive strips
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