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分子生物学
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细胞生物学
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蛋白质组学
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蛋白凝胶纯化
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生化试剂
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仪器设备
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通用实验耗材
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色谱耗材
Product Info Summary
| Species: | Mouse |
|---|---|
| Sample Type: | Serum, Plasma |
| Volume Required per Well: | Min 25 µl |
| Assay Length: | 2.25 hours |
| Specificity: | All analytes < 1% intra-panel cross-reactivity |
| Assay Type: | Planar Based Multiplexed ELISA |
| Detection Method: | Chemiluminescent |
| Multiplex Format: | 96-well solid plate |
| Catalog: | MEK1003 |
| Manufacturer Reference Number: | 110449MS |
| Analytes: | GMCSF, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-6, IL-10, IL-12p70, IL-17, MCP-1, MIP-1α, RANTES, TNFα |
Product Overview
The Multiplex Mouse Cytokine – Inflammation (14-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 14 biomarkers or analytes. Using less than 50 µl of sample per well, up to 80 samples can be assayed for all 14 markers in the panel within 2.25 hours. The Multiplex Mouse Cytokine – Inflammation (14-plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 14 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Assay Ranges
| Units | Calibrator Range | LLD Limit | |
| GMCSF | pg/ml | 2000 - 2.74 | 2.30 |
| IL-1α | pg/ml | 5000 - 6.86 | 4.20 |
| IL-1β | pg/ml | 14000 - 19.20 | 12.41 |
| IL-2 | pg/ml | 5000-6.86 | 3.44 |
| IL-3 | pg/ml | 5000 - 6.86 | 3.18 |
| IL-4 | pg/ml | 5000 - 6.86 | 2.50 |
| IL-6 | pg/ml | 5000 - 6.86 | 2.90 |
| IL-10 | pg/ml | 2000 - 2.75 | 3.00 |
| IL-12p70 | pg/ml | 12000 - 16.46 | 12.20 |
| IL-17 | pg/ml | 10000 - 13.72 | 6.20 |
| MCP-1 | pg/ml | 3000 - 4.12 | 3.40 |
| MIP-1α | pg/ml | 5000 - 6.86 | 0.61 |
| RANTES | pg/ml | 3000 - 4.12 | 2.15 |
| TNFα | pg/ml | 3000 - 4.12 | 3.40 |
Kit Components
Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.
Reagents Include:
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (20): Adhesive strips
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