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产品概况
| 货号 | EK1327 |
|---|---|
| 产品名称 | Rat Adiponectin ELISA Kit |
| 产品描述 | For quantitative detection of rat Adiponectin in , serum, plasma(heparin, EDTA)and urine. |
| 免疫原 | E.coli, I19-N244 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 156pg/ml-10000pg/ml |
| 基因名称 | ADIPOQ |
| 基因全名 | adiponectin, C1Q and collagen domain containing |
| Uniprot ID | Q8K3R4 |
| 指标别名 | 30 kDa adipocyte complement related protein|30 kDa adipocyte complement-related protein|ACDC|ACRP30|ADIPO_HUMAN|Adipocyte|Adipocyte C1q and collagen domain containing protein|Adipocyte complement related 30 kDa protein|Adipocyte complement related protein of 30 kDa|Adipocyte complement-related 30 kDa protein|Adiponectin|Adipoq|Adipose most abundant gene transcript 1|Adipose most abundant gene transcript 1 protein|Adipose specific collagen like factor|ADIPQTL1|ADPN|apM-1|APM1|C1q and collagen domain-containing protein|GBP28|Gelatin binding protein|Gelatin binding protein 28|Gelatin-binding protein |
| 背景资料 | Adiponectin(ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. |
| 显色时间 | 25-30 分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1327-CAP | 96-well plate precoated with anti- rat Adiponectin antibody | 1 |
| EK1327-ST | Lyophilized recombinant rat Adiponectin standard | 10ng/tube×2 |
| EK1327-DA | Biotinylated anti- rat Adiponectin antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 292 | 1705 | 4269 | 265 | 1626 | 4075 |
| Standard deviation | 16.93 | 126.17 | 170.76 | 19.61 | 133.33 | 167.07 |
| CV(%) | 5.8% | 7.4% | 4% | 7.4% | 8.2% | 4.1% |
Rat Adiponectin ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 25-30 分钟)
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.004 | 0.086 | 0.164 | 0.305 | 0.588 | 0.961 | 1.511 | 1.937 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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