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分子生物学
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细胞生物学
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蛋白质组学
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蛋白凝胶纯化
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生化试剂
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仪器设备
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通用实验耗材
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色谱耗材
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产品描述
定量PCR（qPCR，又称Real-time PCR）是一种非常通用的的精确分析基因表达的技术。按方法不同可分为染料法和探针法两类，其中染料法更通用、更方便、成本更低。基于染料的qPCR法通过实时监测结合双链DNA的染料发射的荧光，可以在PCR的每个周期间接测量DNA扩增。当在某一个时间点上，检测到的荧光信号显著超过背景，就可以确定Ct值（Cq值）。所获得的Ct值可用于评估目标基因的相对丰度，也可根据适当的标准曲线计算绝对数量。
我们的产品HotStart™ 2X SYBR Green qPCR Master Mix在定量目标DNA或cDNA方面具有优越的特异性、强劲的扩增效率、理想的可重复性和稳定性。这是一个2X PreMix，使用了结合抗体的热启动Taq DNA聚合酶。理想的Taq聚合酶和合适的缓冲液保证了较好的特异性和较高的扩增速度。Mix中的SYBR Green I可嵌入双链DNA的双螺旋小沟区域，当它与每个周期扩增的双链DNA结合时，会发出绿色荧光，通过仪器监测荧光可以实时的间接定量扩增产物。
该试剂与两种不同浓度的ROX染料一起提供，用于对应的仪器，用来校正仪器中不同反应之间的荧光信号强度。在进行实验时，使用SYBR®或SYBR/FAM模式。
产品特点
· 通过热启动机制优化扩增特异性
· 卓越的灵敏度和准确性
· 在较宽的动态范围内，Ct值具有较高的可重复性
· 方便的2X PreMix，简化实验步骤
组分与储存条件
| Components | 1000 rxn with 10μL reaction 500 rxn with 20μL reaction 200 rxn with 50μL reaction |
5000 rxn with 10μL reaction 2500 rxn with 20μL reaction 1000 rxn with 50μL reaction |
10000 rxn with 10μL reaction 5000 rxn with 20μL reaction 2000 rxn with 50μL reaction |
| HotStart™ 2X SYBR Green qPCR Master Mix | |||
| 50X ROX Reference Dye (low concentration) | |||
| 50X ROX Reference Dye (high concentration) | |||
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将所有组分存放在-20°C并避光保存。尽可能避免反复冻融。 |
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相关生物数据
文献引用
- 1. Runjie Song, Peilan Guo, et al. "A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer." Mol Cancer. 2023 Jul 5;22(1):104. PMID: 37408008
- 2. Xiaotian He, Dechang Zhao, et al. "Intrinsic Immunogenic Tumor Cell Death Subtypes Delineate Prognosis and Responsiveness to Immunotherapy in Lung Adenocarcinoma." Biology 2023, 12(6), 808.
- 3. Junxing Zhao, Zhichao Tang, et al. "Cellular Target Deconvolution of Small Molecules Using a Selection-Based Genetic Screening Platform." ACS Cent Sci. 2022 Oct 26;8(10):1424-1434. PMID: 36313155
- 4. Appolinaire A Olou, Joe Ambrose, et al. "SHP2 regulates adipose maintenance and adipocyte-pancreatic cancer cell crosstalk via PDHA1." J Cell Commun Signal. 2022 Sep 8. PMID: 36074246
- 5. Yang Liu, Libo He, et al. "Pharmacological inhibition of sphingolipid synthesis reduces ferroptosis by stimulating the HIF-1 pathway." iScience. 2022 Jun 6;25(7):104533. PMID: 35784791
- 6. Ziming Li, Tengfei Zhao, et al. "A reactive oxygen species-responsive hydrogel encapsulated with bone marrow derived stem cells promotes repair and regeneration of spinal cord injury." Bioact Mater. 2022 May 9;19:550-568. PMID: 35600969
- 7. Yue Zhuo, Shujun Li, et al. "Targeting SNORA38B attenuates tumorigenesis and sensitizes immune checkpoint blockade in non-small cell lung cancer by remodeling the tumor microenvironment via regulation of GAB2/AKT/mTOR signaling pathway." J Immunother Cancer. 2022 May;10(5):e004113. PMID: 35577506
- 8. Yiting Wan, Yanhua Song, et al. "Upregulated Fibulin-1 Increased Endometrial Stromal Cell Viability and Migration by Repressing EFEMP1-Dependent Ferroptosis in Endometriosis." Biomed Res Int. 2022 Jan 28;2022:4809415. PMID: 35127942
- 9. Guo Z, Liu Y, et al. "Versatile biomimetic cantharidin-tellurium nanoparticles enhance photothermal therapy by inhibiting the heat shock response for combined tumor therapy." Acta Biomater. 2020;110:208-220. PMID: 32278084
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