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分子生物学
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细胞生物学
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蛋白质组学
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蛋白凝胶纯化
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生化试剂
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通用实验耗材
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产品概况
| 货号 | BM1623 |
|---|---|
| 产品名称 | Anti-GAPDH Antibody |
| 基因名 | GAPDH |
| 抗体来源 | mouse |
| 克隆 | Monoclonal(Clone: 5A12) |
| 抗体亚型 | mouse IgG1 |
| 分子量 | 36KD |
| 免疫原 | A synthetic peptide corresponding to a sequence |
| 内容 | 200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA |
| 纯化方式 | protein G purified. |
| 浓度 | 200 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Two transcript variants encoding different isoforms have been found for this gene. |
| Uniprot ID | GAPDH: P04406 |
产品应用细节
为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot: | 1:5000-50000 |
| Immunocytochemistry/Immunofluorescence: | 1:200-500 |
*最优稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
点击图片放大 [list_product_images]Figure 1. Western blot analysis of anti-GAPDH antibody (BM1623). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Testis lysates,Lane 2: Brain lysates,Lane 3: Mouse intestine lysates,Lane 4: Mouse liver lysates,Lane 5: hela whole cell lysates,Lane 6: Colo320 whole cell lysates,Lane 7: Mcf-7 whole cell lysates,Use mouse anti-GAPDH 1:5000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for GAPDH at approximately 36KD. The expected band size for GAPDH is at 36KD.[/list_product_images]
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