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分子生物学
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细胞生物学
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蛋白质组学
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蛋白凝胶纯化
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品名：Anti-LAMA1 Antibody
品牌：博士德
货号：BM1225 -
品名：缺锰改良霍格兰营养液（干粉+浓缩液）
品牌：Coolaber
货号：NSP1020-Mn -
品名：BamHI (10 U/µL)
品牌：ThermoFisher
货号：ER0051/ER0052/ER0055 -
品名：Anti-MYH7 Antibody
品牌：博士德
货号：BM1533 -
品名：Anti-CALM1 Antibody(Monoclonal, 6D4)
品牌：博士德
货号：BM1614 -
品名：AnnexinV Alexa Fluor647/PI 凋亡检测试剂盒
品牌：Solarbio
货号：CA1050 -
品名：DMEM高糖培养基，不含谷氨酰胺
品牌：Gibco
货号：11960044/11960069/11960051/11960077 -
品名：Anti-Insulin-2 Antibody
品牌：博士德
货号：BM0080
产品概况
| 货号 | BM1225 |
|---|---|
| 产品名称 | Anti-LAMA1 Antibody |
| 基因名 | LAMA1; LAMA2; LAMB1; LAMB2 |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal (Clone: LAM-89) |
| 抗体亚型 | Mouse IgG1 |
| 免疫原 | Human laminin. |
| 内容 | 200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. |
| 纯化方式 | Ascites |
| 浓度 | 200ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Laminin is a heterotrimeric extracellular matrix protein consisting of 3 chains: alpha-1,beta-1 and gamma-1, formerly called beta-2(LAMA2). This gene is over 260,000 base pairs and contains 64 exons. Laminin is similar with merosin, a basement membrane-associated protein found in placenta, striated muscle, and peripheral nerve, and both of them are members of the same family of basement membrane proteins. And merosin is the same as laminin M, a striated muscle-specific, basal-lamina-associated protein, it may play a primary role in the pathogenesis of that disorder. |
| 研究类别 | 1. Zhang, X.; Vuolteenaho, R.; Tryggvason, K. : Structure of the human laminin alpha-2-chain gene (LAMA2), which is affected in congenital muscular dystrophy. J. Biol. Chem. 271: 27664-27669, 1996.2. Ehrig, K.; Leivo, I.; Argraves, W. S.; Ruoslahti, E.; Engvall, E. : Merosin, a tissue-specific basement membrane protein, is a laminin-like protein. Proc. Nat. Acad. Sci. 87: 3264-3268, 1990.3. Arahata, K.; Hayashi, Y. K.; Mizuno, Y.; Yoshida, M.; Ozawa, E. : Dystrophin-associated glycoprotein and dystrophin co-localisation at sarcolemma in Fukuyama congenital muscular dystrophy. (Letter) Lancet 342: 623-624, 1993. |
| Uniprot ID | LAMA1; LAMA2; LAMB1; LAMB2: P25391 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Immunohistochemistry(Paraffin-embedded Section): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*最优稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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