货号 | BM1618 |
---|---|
产品名称 | Anti-KRT7 Antibody |
基因名 | KRT7 |
抗体来源 | Mouse |
克隆 | Monoclonal (Clone: LDS-68) |
抗体亚型 | Mouse IgG1 |
分子量 | 51KD |
免疫原 | Cytoskeletal preparation of the RT4 human bladder carcinoma cell line. |
内容 | 200ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol. |
纯化方式 | Ascites |
浓度 | 200 ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | KRT7 is a type II keratin of simple nonkeratinizing epithelia. The deduced 489-amino acid protein has a calculated molecular mass of about 54 kD. K7 contains 4 central alpha-helical segments with heptad repeats of hydrophobic residues characteristic of a coiled-coil region. Within this domain, K7 shares 73% homology with epidermal K6B. KRT7 gene contains 9 exons and spans more than 15.6 kb. K7 gene is mapped to chromosome 12. Keratin 7 is expressed in a wide range of epithelial structures in humans. |
研究类别 | 1. Rosenberg, M.; Fuchs, E.; Le Beau, M. M.; Eddy, R. L.; Shows, T. B. : Three epidermal and one simple epithelial type II keratin genes map to human chromosome 12. Cytogenet. Cell Genet. 57: 33-38, 1991. 2. Smith, F. J. D.; Porter, R. M.; Corden, L. D.; Lunny, D. P.; Lane, E. B.; McLean, W. H. I. : Cloning of human, murine, and marsupial keratin 7 and a survey of K7 expression in the mouse. Biochem. Biophys. Res. Commun. 297: 818-827, 2002. |
Uniprot ID | KRT7: P08729 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
为了提供最优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
---|---|
Western blot: | 1:500-2000 |
Immunohistochemistry(Paraffin-embedded Section): | 1:50-400 |
Immunohistochemistry(Frozen Section): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
*最优稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
Copyright © 2021 新疆宝信源柏生物技术有限公司 版权所有!
新ICP备2022000265号-1 新公网安备 65010502000866号