Anti-CDK4 Antibody(monoclonal, DCS-31)

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货号：BM1572

应用：WB, ICC

反应性：Human, Mouse, Rat

克隆性：单克隆抗体

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SKU	包装规格	单位	目录价	您的价格	库存
BM1572-100ul	100ul	EA	¥1780.00	请登录	请登录
BM1572-150ul	150ul	EA	¥2580.00	请登录	请登录
BM1572-50ul	50ul	EA	¥1060.00	请登录	请登录

产品概况

货号	BM1572
产品名称	Anti-CDK4 Antibody(monoclonal, DCS-31)
基因名	CDK4
抗体来源	Mouse
克隆	Monoclonal (Clone: DCS-31)
抗体亚型	Mouse IgG2a
分子量	34KD
免疫原	Recombinant human Cdk4 protein.
内容	200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol.
纯化方式	Ascites
浓度	200ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	Cyclin-dependent kinase-4(CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. CDK4 expression and activity are required for cytokine responsiveness in T cells.
研究类别	1. Demetrick, D. J.; Zhang, H.; Beach, D. H. : Chromosomal mapping of human CDK2, CDK4, and CDK5 cell cycle kinase genes. Cytogenet. Cell Genet. 66: 72-74, 1994. 2. Modiano, J. F.; Mayor, J.; Ball, C.; Fuentes, M. K.; Linthicum, D. S. : CDK4 expression and activity are required for cytokine responsiveness in T cells. J. Immun. 165: 6693-6702, 2000.
Uniprot ID	CDK4: P11802
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

产品应用细节

为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot:	1:500-2000
Immunocytochemistry:	1:50-400

*最优稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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[list_product_images]Figure 1. Western blot analysis of anti- CDK4 Antibody (BM1572). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: K562 whole cell lysates,
Lane 3: PC-12 whole cell lysates.
Lane 4: NIH/3T3 whole cell lysates,
Lane 5: RAW264.7 whole cell lysates.
Use mouse anti- CDK4 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for CDK4 at approximately 34KD. The expected band size for CDK4 is at 34KD.[/list_product_images]

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