Anti-MYH7 Antibody

货号:BM1533
应用:WB, IHC-P
反应性:Human, Mouse,Rabbit,Rat
克隆性:单克隆抗体
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产品概况

货号BM1533
产品名称Anti-MYH7 Antibody
基因名MYH7
抗体来源Mouse
克隆Monoclonal (Clone: NOQ7.5.4D)
抗体亚型Mouse IgG1
分子量220KD
免疫原Rabbit muscle myosin.
内容200 ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol.
纯化方式Ascites
浓度200 ug/ml
产品形态Liquid
保存条件12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料Myosin is composed of 2 heavy chains of about 200,000 daltons each and 4 light chains of about 20,000 daltons each. Skeletal Myosin(slow),, also known as light chain 3(MYL3), mapped to 3p. Fodor et al.(1989) found that the MYL3 gene has 7 exons, the last of which is completely untranslated 3-prime sequence.
研究类别1. Darras, B. T.; Fodor, B.; Vanin, E.; Francke, U. : A human myosin alkali light chain gene mapped to chromosome 3. (Abstract) Cytogenet. Cell Genet. 46: 603, 19872. Fodor, W. L.; Darras, B.; Seharaseyon, J.; Falkenthal, S.; Francke, U.; Vanin, E. F. : Human ventricular/slow twitch myosin alkali light chain gene characterization, sequence, and chromosomal location. J. Biol. Chem. 264: 2143-2149, 1989.
Uniprot IDMYH7: P12883
推荐配套的二抗和检测试剂Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

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[list_product_images]Figure 1. Western blot analysis of MYH7 using anti- Myosin(Skeletal, Slow) antibody (BM1533).
The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse skeletal muscle tissue lysates,
Lane 2: rat skeletal muscle tissue lysates.
Use mouse anti- MYH7 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for MYH7 at approximately 220KD. The expected band size for MYH7 is at 223KD.[/list_product_images]
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