Anti-HSPD1 Antibody

产品中心

分子生物学
细胞生物学
蛋白质组学
蛋白凝胶纯化
生化试剂
仪器设备
通用实验耗材
- 塑料制品
  - 吸头（盒）
  - 离心管
  - PCR管
  - 八连管
  - 冻存管（盒）
  - 光学&反应板
  - 封板膜
  - 酶标板
  - 巴氏吸管
  - Transwell嵌套
  - PCR板
  - 梳子
  - 深孔板
  - 培养皿
  - 切片盒
  - 晾片架
  - 孵育盒
  - 培养管
  - 加样槽
  - 移液器架
  - 洗瓶
  - 湿盒
  - 摇瓶
  - 涂布棒
  - 真空过滤器
  - 培养液瓶
  - 微孔板
  - 滚瓶
  - 容量瓶
  - 量筒
  - 烧杯
  - 储液桶
- 玻璃制品
  - 玻璃培养皿
  - 玻璃烧杯
  - 玻璃量筒
  - 玻璃容量瓶
  - 玻璃移液管
  - 玻璃漏斗
  - 载玻片
  - 试剂瓶
  - 滴定管
  - 玻璃板
  - 玻底皿
  - 刻度移液管
  - 锥形瓶
  - 比重计
  - 广口瓶
  - 滴瓶
  - 盖玻片
  - 烧瓶
  - 蒸发皿
- 防护用品
- 过滤产品
  - 滤器
  - PVDF膜/NC膜
  - 滤膜
  - 滤纸
  - 超滤离心管
  - 抽滤瓶
- 其他实验耗材
  - 干燥器
  - 其他耗材
  - 染色缸/架
  - 冰盒
  - 细胞筛网
  - 自封袋
  - 称量用品
  - 搅拌棒/搅拌子
  - 程序降温盒
  - 比色皿
  - 磁力架
  - 透析袋
  - 封口膜
  - 接种环/针
  - 研磨棒
  - 采样管
  - 离心管架/盒
  - 离心瓶
  - 发光板
  - 检测板
  - 刀片
  - 细胞计数板
  - 胖肚移液管
  - 药敏纸片
  - 铝块
- 临时耗材
色谱耗材

浏览历史

货号：BM1511

应用：WB, IHC-P

反应性：Human, Mouse, Rat, Chicken

克隆性：单克隆抗体

收藏产品大包装咨询？

SKU	包装规格	单位	目录价	您的价格	库存
BM1511-100ul	100ul	EA	¥1780.00	请登录	请登录
BM1511-150ul	150ul	EA	¥2580.00	请登录	请登录
BM1511-50ul	50ul	EA	¥1060.00	请登录	请登录

产品概况

货号	BM1511
产品名称	Anti-HSPD1 Antibody
基因名	HSPD1
抗体来源	Mouse
克隆	Monoclonal (Clone: LK1)
抗体亚型	Mouse IgG1
分子量	68KD
免疫原	Recombinant human heat shock protein 60(HSP60)
内容	200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol.
纯化方式	Ascites
浓度	200 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	Heat shock 60KD protein(HSP60) is a member of the chaperonin class of protein factors and the nuclear encoded mitochondrial HSP60 is required for the assembly into oligomeric complexes of proteins imported into the mitochondrial matrix. HSP60 is linked head to head comprising approximately 17 kb and consist of 12 exons. HSP60 is a self-molecule, it can downregulate adaptive immune responses by upregulating Tregs innately through TLR2 signaling.
研究类别	1. Cheng, M. Y.; Hartl, F.-U.; Martin, J.; Pollock, R. A.; Kalousek, F.; Neupert, W.; Hallberg, E. M.; Hallberg, R. L.; Horwich, A. L. : Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria. Nature 337: 620-625, 1989.2. Hansen, J. J.; Bross, P.; Westergaard, M.; Nielsen, M. N.; Eiberg, H.; Borglum, A. D.; Mogensen, J.; Kristiansen, K.; Bolund, L.; Gregersen, N. : Genomic structure of the human mitochondrial chaperonin genes: HSP60 and HSP10 are localised head to head on chromosome 2 separated by a bidirectional promoter. Hum. Genet. 112: 71-77, 2003. Note: Erratum: Hum. Genet. 112: 436 only, 2003.3. Zanin-Zhorov, A.; Cahalon, L.; Tal, G.; Margalit, R.; Lider, O.; Cohen, I. R. : Heat shock protein 60 enhances CD4+CD25+ regulatory T cell function via innate TLR2 signaling. J. Clin. Invest. 116: 2022-2032, 2006.
Uniprot ID	HSPD1: P10809
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

产品应用细节

为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot:	1:500-2000
Immunohistochemistry(Paraffin-embedded Section):	1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*最优稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

点击图片放大

[list_product_images]igure 1. Western blot analysis of anti- HSPD1 antibody (BM1511).The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1:Human HELA whole cell lysates,
Lane 2:U87 whole cell lysates,
Lane 3: Rat brain tissue lysates,
Lane 4: Neuro-2a whole cell lysates.
Use rabbit anti- HSPD1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HSPD1 at approximately 68KD. The expected band size for HSPD1 is at 61KD.[/list_product_images]

暂无产品信息

暂无参考文献

暂无文件下载