货号 | BM0096 |
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产品名称 | Anti-Myosin(Skeletal, Fast)MYH1/MYH2 Antibody (Monoclonal, MY-32) |
基因名 | MYH1; MYH2 |
抗体来源 | Mouse |
克隆 | Monoclonal (Clone: MY-32) |
抗体亚型 | Mouse IgG1 |
分子量 | 230KD |
免疫原 | Rabbit muscle myosin. |
内容 | 200 ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol. |
纯化方式 | Ascites |
浓度 | 200 ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | Myosin is composed of 2 heavy chains of about 200,000 daltons each and 4 light chains of about 20,000 daltons each. The light chains are of 2 distinct types: the phosphorylatable, regulatory, or MLC2 type, and the nonphosphorylatable, alkali, or MLC1 and MLC3 types(MYL1 according to the HGM symbols). Skeletal Myosin(Fast),, also known as MYL1, mapped to the region 2q32.1-qter. |
研究类别 | 1. Cohen-Haguenauer, O.; Barton, P. J. R.; Van Cong, N.; Serero, S.; Gross, M.-S.; Jegou-Foubert, C.; de Tand, M.-F.; Robert, B.; Buckingham, M.; Frezal, J. : Assignment of the human fast skeletal muscle myosin alkali light chains gene (MLC1F/MLC3F) to 2q32.1-2qter. Hum. Genet. 78: 65-70, 1988. 2. Serero, S.; Barton, P.; Van Cong, N.; Cohen-Haguenauer, O.; Robert, B.; Buckingham, M.; Frezal, J. : Assignment of the human fast skeletal muscle myosin alkali light chains gene (MLC1F/MLC3F) to 2q32.1-2qter. (Abstract) Cytogenet. Cell Genet. 46: 690 only, 1987. |
Uniprot ID | MYH1; MYH2: P12882; Q9UKX2 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
为了提供最优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Western blot: | 1:500-2000 |
Immunohistochemistry(Paraffin-embedded Section): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
*最优稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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