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分子生物学
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细胞生物学
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蛋白质组学
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蛋白凝胶纯化
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品名：Anti-IVL Antibody
品牌：博士德
货号：BM1217 -
品名：Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)
品牌：APExBIO/艾普拜
货号：APE-K1010 -
品名：BpiI (BbsI) (10 U/µL)
品牌：ThermoFisher
货号：ER1011/ER1012 -
品名：Annexin V-Biotin Apoptosis Kit
品牌：APExBIO
货号：APE-K2009 -
品名：Goat Anti-Human IgG Fc (FITC)
品牌：abcam
货号：ab97224 -
品名：Annexin V-Cy5 Apoptosis Kit
品牌：APExBIO/艾普拜
货号：APE-K2005 -
品名：Cfr9I (XmaI) (10 U/µL)
品牌：ThermoFisher
货号：ER0171/ER0172 -
品名：Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc - BSA and Azide free
品牌：abcam
货号：ab288280
产品概况
| 货号 | BM1217 |
|---|---|
| 产品名称 | Anti-IVL Antibody |
| 基因名 | IVL |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal (Clone: SY5) |
| 抗体亚型 | Mouse IgG1 |
| 分子量 | 68KD |
| 免疫原 | Human involucrin. |
| 内容 | 200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. |
| 纯化方式 | Ascites |
| 浓度 | 200ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Involucrin is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. The gene consists of 585 amino acids, 390 of which form a central decapeptide repeat, rich in glutamine and glutamic acid. The involucrin gene, encoding a protein of the terminally differentiated keratinocyte, is polymorphic in the human. Involucrin has previously been mapped to chromosome 1q21. The epidermal protein involucrin has been remodeled in the higher primates. And the existence of two populations of East Asian involucrin alleles gives support for the existence of a Eurasian stem lineage from which Caucasians and a part of the Asian population originated. |
| 研究类别 | 1. Eckert, R. L.; Green, H. : Structure and evolution of the human involucrin gene. Cell 46: 583-589, 1986.2. Djian, P.; Delhomme, B.; Green, H. : Origin of the polymorphism of the involucrin gene in Asians. Am. J. Hum. Genet. 56: 1367-1372, 1995. |
| Uniprot ID | IVL: P07476 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot: | 1:500-2000 |
| Immunohistochemistry(Paraffin-embedded Section): | 1:50-400 |
| Immunohistochemistry(Frozen Section): | 1:50-400 |
| Immunocytochemistry: | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*最优稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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