Anti-LMNA Antibody

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品名：Anti-LMNA Antibody
品牌：博士德
货号：BA1227

货号：BA1227

应用：WB, IHC-P, ICC

反应性：Human, Mouse, Rat

克隆性：内参抗体

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SKU	包装规格	单位	目录价	您的价格	库存
BA1227-100ul	100ul	EA	¥880.00	请登录	请登录
BA1227-1ml	1ml	EA	¥6800.00	请登录	请登录
BA1227-200ul	200ul	EA	¥1680.00	请登录	请登录

产品概况

货号	BA1227
产品名称	Anti-LMNA Antibody
基因名	LMNA
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit IgG
分子量	70-74KD
免疫原	A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin A/C(455-469aa RNKSNEDQSMGNWQI), identical to the related rat and mouse sequences.
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	Immunogen affinity purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	Lamins are structural protein components of the nuclear lamina, a protein network underlying the inner nuclear membrane that determines nuclear shape and size. There are three types of lamins, A,B and C. The lamin A/C(LMNA) gene contains 12 exons. Alternative splicing within exon 10 gives rise to two different mRNAs that code for pre-lamin A and lamin C. Lamin A/C mapped to 1q21.2-q21.3 and mutations in this gene cause a variety of human diseases including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and Hutchinson-Gilford progeria syndrome. Lamin A/C deficiency is thus associated with both defective nuclear mechanics and impaired mechanically activated gene transcription.
研究类别	1. Lammerding, J.; Schulze, P. C.; Takahashi, T.; Kozlov, S.; Sullivan, T.; Kamm, R. D.; Stewart, C. L.; Lee, R. T. : Lamin A/C deficiency causes defective nuclear mechanics and mechanotransduction. J. Clin. Invest. 113: 370-378, 2004.2. Lin, F.; Worman, H. J. : Structural organization of the human gene encoding nuclear lamin A and nuclear lamin C. J. Biol. Chem. 268: 16321-16326, 1993.3. Wydner, K. L.; McNeil, J. A.; Lin, F.; Worman, H. J.; Lawrence, J. B. : Chromosomal assignment of human nuclear envelope protein genes LMNA, LMNB1, and LBR by fluorescence in situ hybridization. Genomics 32: 474-478, 1996.
Uniprot ID	LMNA: P02545
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。

产品应用细节

为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot:	1:500-2000
Immunohistochemistry(Paraffin-embedded Section):	1:50-400
Immunocytochemistry:	1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*最优稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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[list_product_images]Figure 1. Western blot analysis of anti- LMNA antibody (BA1227).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Human placenta tissue lysates,Lane 2: Human PC-3 whole cell lysates,Lane 3: Human HELA whole cell lysates,Lane 4: Human HepG2 whole cell lysates,Lane 5: Human CACO-2 whole cell lysates,Lane 6: rat spleen tissue lysates,Lane 7: mouse liver tissue lysates.Use rabbit anti- LMNA 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for LMNA at approximately 70-74KD. The expected band size for LMNA is at 74KD.|IHC(P): Human Lung Cancer Tissue |IHC(P): Mouse Intestine Tissue|IHC(P): Rat Intestine Tissue [/list_product_images]

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