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分子生物学
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细胞生物学
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蛋白质组学
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蛋白凝胶纯化
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品名：Anti-MAPK1 Antibody
品牌：博士德
货号：BM1595 -
品名：Anti-CDK6 Antibody(monoclonal, DCS-90)
品牌：博士德
货号：BM1616 -
品名：CseI (HgaI) (5 U/µL)
品牌：ThermoFisher
货号：ER1901 -
品名：Recombinant Human TMEM59 Protein (His Tag)
品牌：abcam
货号：ab314625 -
品名：BseSI (Bme1580I) (10 U/µL)
品牌：ThermoFisher
货号：ER1441 -
品名：BAFF, human recombinant protein
品牌：APExBIO
货号：APE-P1044 -
品名：Cla I
品牌：TaKaRa
货号：1034 -
品名：DpnI (10 U/µL)
品牌：ThermoFisher
货号：ER1701/ER1702/ER1705
产品概况
| 货号 | BM1595 |
|---|---|
| 产品名称 | Anti-MAPK1 Antibody |
| 基因名 | MAPK1; MAPK3 |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal (Clone: MAPK-YT) |
| 抗体亚型 | Mouse IgG1 |
| 分子量 | 43KD |
| 免疫原 | A synthetic peptide containing 11 amino acids, HTGFLTpEYpVAT, corresponding to the phosphorylated form of ERK-activation loop conjugated to KLH. |
| 内容 | 200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. |
| 纯化方式 | Ascites |
| 浓度 | 200ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | In mammalian cells, a variety of extracellular stimuli generate intracellular signals that converge on a limited number of so-called mitogen-activated protein(MAP) kinase pathways. The central core of each MAP kinase(MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase(MAPKKK) phosphorylates and activates a specific MAPK kinase(MAPKK), which then activates a specific MAPK. Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome. |
| 研究类别 | 1. Rodriguez-Viciana, P.; Tetsu, O.; Tidyman, W. E.; Estep, A. L.; Conger, B. A.; Santa Cruz, M.; McCormick, F.; Rauen, K. A. : Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome. Science 311: 1287-1290, 2006. 2. Scholl, F. A.; Dumesic, P. A.; Barragan, D. I.; Harada, K.; Bissonauth, V.; Charron, J.; Khavari, P. A. : Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Dev. Cell 12: 615-629, 2007. |
| Uniprot ID | MAPK1; MAPK3: P28482 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot: | 1:500-2000 |
| Immunohistochemistry(Paraffin-embedded Section): | 1:50-400 |
| Immunocytochemistry: | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*最优稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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